Most of the stations aired two episodes each week, usually on the weekends and many times back to back.Many of the stories are based on Rod Serling's scripts from the original Twilight Zone series, and are slightly expanded and updated to reflect contemporary technology and trends and the lack of a visual component. In addition to adapting all of the original episodes aired on the TV series, the radio series has also adapted some Twilight Zone TV scripts which were never produced, scripts from other Serling TV productions, and new stories written especially for the radio series.

Taking Serling's role as narrator is Stacy Keach. Different Hollywood actors, such as Blair Underwood and Jim Caviezel, take the lead role in each radio drama. In addition, several stars who appeared on the original TV series, such as H.M. Wynant, Orson Bean and Morgan Brittany, appear, although purposely not in the roles they originated on television. The series features a full cast, music and sound effects and is produced in the flavor of classic radio dramas but using today's technology.

In addition to being an homage to the original Twilight Zone TV series, the radio dramas pay tribute to the era of classic radio drama, including allusions to radio dramas such as Gunsmoke, the presence of radio legend Stan Freberg in many episodes, and the sons (Stacy Keach, Ed Begley Jr.) of radio drama personalities Stacy Keach Sr. and Ed Begley as stars in the series.