“To retain antibody bioactivity, mild, near-physiological conditions are often used for conjugation reactions. Under these conditions, endogenous amino acids such as Lys and Cys are chemically reactive and can be used as conjugation sites. We made a great effort to complete this complicated and difficult task by engineering special moieties with unique dar conjugation chemistries into antibody sequences in our antibody design services.” A scientist from Creative Biolabs said that.
“Alongside the efforts of antibody engineering, we also apply several enzymatic or physical approaches to afford site-specific anti drug conjugate by modifying the carbohydrate moiety via galactosidases and galactosyltransferases or photo-crosslinking indole-3-butyric acid containing linkers onto antibody nucleotide binding site.”
https://www.creative-biolabs.com/adc/antibody-design-and-conjugation.htm