Antibody-drug conjugates (ADCs) are a class of drugs that conjugate a monoclonal antibody to a different number of small molecule cytotoxins (effector molecules) by chemical linkers. This novel drug combines the high specificity of monoclonal antibodies with the high activity of small molecule toxins.
Chromatographic analysis of antibody-conjugated drugs and their enzymatic products can obtain important information such as the number and location of coupling sites, free drug content, and drug-antibody coupling ratio. At the same time, chromatographic separation techniques can purify different components for further analysis. Chromatographic techniques based on hydrophobic, charge and molecular size principles have been widely used to analyze antibody conjugation.
https://www.creative-biolabs.com/adc/antibody-design-and-conjugation.htm